Figure 3.

Characterization of cells infiltrating kidneys of CfH^−/− MRL-lpr mice. (A) Representative immunohistochemical staining showing marked glomerular and periglomerular accumulation of 7/4⁺ (b) and CD11b⁺ (d) cells, as well as periglomerular and perivascular accumulation of CD4⁺ and F4/80⁺ cells (f and h) in the kidneys of CfH^−/− MRL-lpr mice compared with control CfH^+/+ MRL-lpr mice (a, c, e, and g). Original magnifications, ×400. (B) Flow cytometric analyses of CD11b⁺ cells from kidneys of 12-week-old MRL-lpr mice, showing greater quantities of infiltrating CD11b⁺ cells in CfH^−/− MRL-lpr mice compared with CfH^+/+ MRL-lpr mice. CD11b⁺ cells from the kidneys of CfH^−/− MRL-lpr mice were further analyzed and found to consist of three distinct populations of CD11b⁺Gr1^highF4/80⁻, CD11b⁺Gr1^interF4/80⁺, and CD11b⁺Gr1^lowF4/80⁻ cells. CD11b⁺F4/80⁺ cells also had higher CD11c expression than that of CD11b⁺Gr1^lowF4/80⁻ cells. (C) The activation status of renal infiltrating CD4⁺ cells was evaluated by the relative quantities of CD44^highCD62L^low, CD69⁺, and CD25⁺ cells. The numbers in the two-dimensional histograms correspond to the percentage of cells contained within the adjacent red boundary. SSC, side scatter.