Figure 12.

Effects of zinc oxide (ZnO) nanoparticles (NPs) on changes in mitochondrial activity of minimal defined serum-free medium (MDSF) (A)- or MDSF supplemented with 10% newborn bovine serum (MDS) (B)-grown rat alveolar epithelial cell monolayers (RAECMs). Monolayers were exposed apically to ZnO NPs (up to 176 μg/ml) for 24 hours before 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Positive control was monolayers exposed to 0.1% Triton X-100 for 15 minutes. Negative control was monolayers not exposed to ZnO NPs. *Significantly different (P < 0.05) from negative control.