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. 2010 Jul 16;182(11):1398–1409. doi: 10.1164/rccm.201002-0185OC

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Figure 2. — Effects of washout of zinc oxide (ZnO) nanoparticles (NPs) (176 μg/ml) at 1, 3, and 7 hours after NPs instillation by replacing apical fluid with fresh minimal defined serum-free medium (MDSF) (A) or MDSF supplemented with 10% newborn bovine serum (MDS) (B). R_T of MDSF- and MDS-grown rat alveolar epithelial cell monolayers (RAECMs) before ZnO NP exposure was 2.12 (±0.21) kΩcm² (n = 9) and 2.46 (±0.71) kΩcm² (n = 18), respectively. Arrow indicates the time when apical fluid was replaced with fresh culture medium. *Significantly different (P < 0.05) from control (RAECMs not exposed to ZnO NPs).