Figure 3.

Role of chitin in sensitization to ovalbumin (OVA) in vivo. MyD88 and Toll-like receptor 2 (TLR-2) sufficient (+/+; +) and deficient (−/−; −) mice were sensitized by two intraperitoneal injections (Days 0 and 5) of a mixture containing OVA (20 μg), chitin (25 μg), OVA complexed with chitin, or their vehicle controls. One week later the mice received aerosolized OVA for 3 days and 1 day after the last challenge were killed and serum was obtained. The levels of (A) total and (B) OVA-specific IgE were assessed by ELISA. Results are presented as the mean ± SEM of a minimum of six mice per group. ***P < 0.001.