Figure 7.

Roles of IL-17A in the adjuvant effects of chitin in vivo. Wild-type and IL-17A^−/− mice were sensitized with OVA and chitin and then challenged with ovalbumin (OVA). (A) Total bronchoalveolar lavage (BAL) cell and (B) eosinophil recovery, (C) tissue inflammation (hematoxylin and eosin, ×20 final magnification), and mucus metaplasia (periodic acid-Schiff [PAS], ×40 final magnification), and the levels of (D) BAL IL-4, (E) IL-5, and (F) IL-13 were evaluated. Supernatant cytokines were measured using ELISA. The results are expressed as the mean ± SEM of a minimum of six mice per group. ***P < 0.001. The arrow in C indicates PAS-positive airway mucus.