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. 2010 Jul 23;182(12):1516–1523. doi: 10.1164/rccm.201003-0452OC

Figure 7.

Figure 7.

Urokinase-type plasminogen activator (uPA) and milk fat globule epidermal growth factor-8 (MFG-E8) have competitive effects on efferocytosis. (A) uPA diminishes the enhanced effect of MFG-E8 on phagocytosis of apoptotic neutrophils. Macrophages were preincubated with human serum albumin (HSA) or single-chain plasminogen activator (scuPA) (1 μg/ml) for 1 hour at 37°C in RPMI medium. The cells were then washed three times and incubated with HSA or MFG-E8 (1 μg/ml) for an additional 1 hour at 37°C. After washing three times, apoptotic neutrophils were added to the cultures and phagocytosis assays were performed. (B) The enhancement of phagocytosis induced by preincubation of macrophages with MFG-E8 is not affected by the subsequent addition of uPA. Macrophages were preincubated with HSA or MFG-E8 (1 μg/ml) for 1 hour at 37°C in RPMI medium. The cells were then washed three times and incubated with HSA or uPA (1 μg/ml) for an additional 1 hour at 37°C. After washing three times, apoptotic neutrophils were added to the cultures and phagocytosis assays were performed. Data shown are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.