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. 2010 Aug 6;182(12):1546–1553. doi: 10.1164/rccm.200912-1888OC

Figure 8.

Figure 8.

Electrophoresis of polymerase chain reaction (PCR) products amplified with mycobacteria-specific primers. After PCR using chromosomal DNA as the template and specific primers, DNAs were separated in 2% agarose gel electrophoresis followed by ethidium bromide staining. Observation of a 435-bp PCR indicates the presence of mycobacteria. M, DNA size marker 100 bp (Bio-Rad, Hercules, CA); lane 1, RAW 264.7 cells without Mycobacterium avium; lane 2, RAW 264.7 cells incubated with M. avium for 24 hours (4× stock); lane 3, RAW 264.7 cells incubated with M. avium for 24 hours (2× stock); lane 4, RAW 264.7 cells loaded with amikacin–fluorescein isothiocyanate (FITC) (8 mg/L) and incubated with M. avium for 24 hours; lane 5, RAW 264.7 cells loaded with amikacin-FITC (64 mg/L) and incubated with M. avium for 24 hours; lane 6, H2O.