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. 2011 Jan 19;7(1):87–101. doi: 10.7150/ijbs.7.87

Figure 2.

Figure 2

HOXA3 expression during branchial nerve development. (A) At st-18 of chicken embryos, strong HOXA3 expression along the IXth and Xth nerves is observed. The geniculate placode expresses HOXA3 (large white arrow). (B) At st-11, HOXA3 is expressed in the region posterior to the r4/5 boundary and in NCCs from r6 (arrowheads). In situ hybridization of st-15 embryos for HOXA3 (C) and PHOX2B (D) shows that HOXA3 is expressed in the area including placode-derived cells of the IXth and Xth nerve and in the geniculate placode (large white arrows). VII, IX, and X indicate the precursor cells of the VIIth, IXth, and Xth nerves, respectively. (E,F) Transverse sections of st-12 at r4 (E) and at r6 (F). At r4, HOXA3 is weakly expressed with a slightly stronger signal in the dorsal region (E). At the r6 level, HOXA3 signal is observed in the neural tube, and strongly in NCCs (F, arrowheads). OV, otic vesicle.