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. 2011 Jan 19;7(1):87–101. doi: 10.7150/ijbs.7.87

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Electroporation of morpholino oligo into the neural tube and NCCs. (A) Antisense oligo is detected in the cellular streams to branchial arches 2,3, and 4 (ba2,3,4). (B) Staining for HNK-1 in the embryo shown in “A” confirms that these streams are NCCs. (C,D) At st-19+, control (C) or antisense (D) morpholino oligo is detected around the distal ganglia of the VIIth, IXth, and Xth nerves (arrows). (E,F) In embryos electroporated with control (E) or antisense (F) oligo, SOX10-expressing cells are observed around the distal ganglia of the VIIth, IXth, and Xth nerve (arrowheads). In “F,” the VIIth nerve connection is disrupted (bracket).