Figure 6.

Electroporation of morpholino oligo into the neural tube and NCCs. Electroporation with control (A,C,E,G) or antisense (B,D,F,H) morpholino was performed, and embryos were stained for ISLET-1 and neurofilaments. Double fluorescence images (C,D,G,H) and single fluorescence ones for ISLET-1 (A,B,E,F) are shown. Dorsal migration of the placode-derived cells of the VIIth, IXth, and Xth nerves (arrowheads) is revealed (A,E), but migration is affected in placode-derived cells of the VIIth (bracket in “B”) and Xth nerve (arrowheads in “F”). The VIIth nerve fiber in the embryo electroporated with antisense oligo (arrow in “D”) is thinner than that in the control embryo (arrow in “C”), and the Xth nerve connection is disrupted (bracket in “H”). Large arrowheads (C,D,G,H) indicate the IXth nerve fiber. XII in “H” indicates the XIIth nerve.