Figure 6.

(a) Mean buried area (MBA) of the apoMb₁₁₉ sequence calculated according to Rose et al. (71). High scores correspond to nonpolar regions of the chain. (b) Normalized experimental binding affinities of full-length DnaK (dashed line) and DnaK-β (solid line) for the apoMb₁₁₉ sequence (72). (Shaded areas) Chain regions corresponding to the assigned backbone residues. (Solid bars above the plot) Position of the native α-helices in full-length apoMb. (c) ¹H,¹⁵N-HSQC spectrum of ¹⁵N-DnaK-β chaperone in the absence and presence of variable amounts of unlabeled apoMb₁₁₉, in 10 mM sodium acetate and 5% D₂O, at pH 6.0. Spectra acquired at different stoichiometric ratios are color-coded according to the scheme provided in the legend. Specific resonance shifts observed upon addition of increasing amounts of apoMb₁₁₉ (indicated by arrows to guide the eye).