Figure 5.

Cross-linking of IAPP and insulin bound to POPG/POPC LUVs. IAPP (25 μM) or IAPP/insulin (25 μM/25 μM) was incubated with POPG/POPC vesicles (1:1 molar ratio, 200 μM) at room temperature for the indicated time points. At each time point, aliquots of each sample were removed and cross-linked with 0.01% (v/v) glutaraldehyde. The reactions were quenched with the addition of lysine and loading buffer, boiled, and separated by SDS-PAGE. The gel was transferred and then immunoblotted with a rabbit polyclonal α-IAPP antibody.