Figure 10.

Role of AC5 and phospho-ERK on dark-induced crf expression. Expression of crf in dark-reared wild-type siblings (A), dark-reared fantasma mutant (B), and dark-reared wild type treated with 50 μm ΜΕK inhibitor PD98059 (C). Note that the light brown spots on the images are melanocytes containing melanin. Melanocytes fail to undergo melanosome dispersal in the fan mutant; therefore, there are fewer such brown spot-covered areas in the fan mutant in B. D, Quantification of ratio of intensity normalized to respective light-reared controls shows that both the fan mutation and PD98059 treatment dampen dark-induced crf expression, with the exception of the hindbrain cluster in PD98059 treatment. ***p < 0.001, **p < 0.01, *p < 0.05, n = 10 one-way ANOVA applied to individual clusters to compare with WT dark control. Colored circles and abbreviations: Red, Telencephalon (Tel); light blue, pretectum (PreT); black, preoptic (Po); green, posterior tuberculum (PT); pink, hypothalamus (Hy); dark blue, hindbrain (HB).