FIGURE 2.

C. maenas hemoglobin is a gill-specific protein. About 70 μg hemolymph proteins and protein extracts from whole tissue were separated by SDS-PAGE, transferred onto a nitrocellulose membrane, and stained with purified polyclonal anti-CmaHb antibodies. A, CmaHb was detected in the fraction containing proteins from whole tissue. B, RNA and protein were isolated from the indicated crab tissues. CmaHb mRNA was determined by RT-PCR (B, upper row). Western blotting experiments were performed with ∼70 μg total proteins of tissue extracts (B, lower row). CmaHb protein and mRNA are restricted to the gills.