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Localization of C. maenas hemoglobin in gills. An affinity purified anti-CmaHb antibody was applied on cryosections of gills. CmaHb immune reactivity was found at the membrane of chief cells in the lateral area of the central stem (A) and at the membrane of cells of the lamellae (C). In preabsorption tests no specific immune staining but only background fluorescence was observed (B). cc, chief cells; gc, gill chamber; hl, hemolymph; pc, pillar cells.
