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Subcellular localization of hemoglobin by Western blot analysis. About 70 μg protein extract isolated from four different cellular compartments were applied on each lane and used for Western blotting. CmaHb protein was mainly detected in the fraction that includes the membrane proteins. An additional weak signal was observed in the cytosolic fraction, whereas no CmaHb protein was detectable in the protein samples containing nuclear proteins and the residual ones.
