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. 2010 Dec 2;286(5):3242–3249. doi: 10.1074/jbc.M110.150300

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Polyubiquitination profiles of TRAF6. The levels of Lys⁶³- and Lys⁴⁸-specific polyubiquitination (Ub) were investigated at day 10 by Western blotting (IB) after immunoprecipitation (IP) of TRAF6. A, polyubiquitination profiles in M-CSF/sRANKL (MR), M-CSF/LPS added at day 7 (ML) and M-CSF/sRANKL/LPS added at day 7 (MRL). **, p < 0.01 compared with MR; ##, p < 0.01 compared with ML. B, polyubiquitination profiles of PBMCs transfected with either nontargeting (NC) or A20 siRNAs ± LPS. Results were expressed as a specific ubiquitination:total TRAF6 ratio. **, p < 0.01 compared with untreated cells; ##, p < 0.01 compared with nontargeting siRNA. Error bars = S.E.