FIGURE 6.

LPS treatment of osteoclast cultures resulted in the inhibition of the NF-κB pathway. A, PBMCs were cultured in the presence of LPS, and the phosphorylation of IκB was assessed by Western blotting at day 8 (d8) and day 10 (d10), 30 min after the culture medium was replenished with fresh culture medium. B, PBMCs were transfected with nontargeting siRNAs (NC) or siRNAs targeting A20 (A20) and cultured in the presence of M-CSF/LPS. The extent of IκB phosphorylation was assessed at day 10, 30 min after the medium was replenished. **, p < 0.01 compared with day 8; ##, p < 0.01 compared with nontransfected cells. Error bars = S.E.