FIGURE 7.

Targeting A20 resulted in restoration of TRAF6 and increased bone resorption in M-CSF/LPS-treated cultures. A, PBMCs were transfected with either nontargeting siRNAs (NC) or siRNAs targeting A20 and cultured in the presence of M-CSF and LPS. The expression of A20 and TRAF6 was assessed by Western blotting at day 10. B, the number of osteoclasts was assessed by counting the number of TRAP-positive cells at day 14. C, expressions of cathepsin K (Cath.K), OSCAR, and calcitonin receptor (CT-R) at day 14 were assessed in the absence of A20 with or without LPS stimulation. D, extent of lacunar resorption was assessed after 21 days. **, p < 0.01 compared with nontransfected cells. Error bars = S.E.