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. 2010 Nov 18;286(5):3250–3260. doi: 10.1074/jbc.M110.157545

FIGURE 1.

FIGURE 1.

Hoxa1 is transcriptionally activated by RA in WT and RARβ/KO but not in RARγ/KO and RARE/KO cells. A, schematic of the Hoxa1 3′-RARE targeting construct. B, Northern blot showing dose response of Hoxa1 induction 48 h after different doses of RA in WT, heterozygous RARE/KO, and two independently derived lines with RARE/KO in both alleles. C, relative Hoxa1 expression prior to and 24 h after 1 μm RA in WT, RARE/KO, and RARγ/KO ESCs. Quantitative RT-PCR was used to quantify transcripts normalized to a 36B4 reference control (mean ± S.E.). Asterisk denotes p < 0.05 for the relative expression of Hoxa1 mRNA in WT compared with the RARE/KO and RARγ/KO ESCs. D, relative Hoxa1 expression prior to and 24 h after 1 μm RA in WT and RARβ/KO ESCs. Quantitative RT-PCR was used to quantify transcripts normalized to a 36B4 reference control (mean ± S.E.).