FIGURE 7.
Tandem MS analysis of lipid extracts of purified cotton root lipid droplets. In addition to tandem MS of LDs sampled through DOMS, precursor-product scans of lipid extracts of purified LDs of cotton roots aided in identification of cyclic fatty acids. Shown are precursors TAG profiles of malvalic/linoleic (a), sterculic (b), and dihydrosterculic (c) fatty acids analyzed in neutral loss mode on a triple quadrupole MS. Dominant TAG species are identified as ammonium adducts [M + NH4]+ with peaks labeled according to the total number of carbons followed by the total number of double bonds for that particular TAG mass-to-charge ratio. Underlined species in the malvalic/linoleic scan (a) correspond to TAGs with at least one sterculic or dihydrosterculic acyl chain.