TABLE 1.
Innate immune parameters elicited by LM infection
| LIMP-2−/− | Wild type | |
|---|---|---|
| Sera cytokine concentrationa | ||
| TNF-α | 309.5 ± 11 | 3.500 ± 35 |
| MCP-1 | 612.5 ± 13 | 2.803 ± 21 |
| IL-6 | 809.5 ± 12 | 4.500 ± 45 |
| IL-12 | 34.1 ± 5 | 31.2 ± 8 |
| IL-10 | 5.1 ± 1 | 6.2 ± 2 |
| IFN-γ | 177.3 ± 12 | 163.45 ± 13 |
| BM-DM cytokine productionb | ||
| TNF-α/LMwt | 816.8 ± 13 | 5101.2 ± 21 |
| MCP-1/LMwt | 942.1 ± 25 | 4619.8 ± 16 |
| IL-6/LMwt | 2909 ± 11 | 6502 ± 14 |
| IL-10/LMwt | 189 ± 5 | 193 ± 2 |
| TNF-α/LMhly− | 140 ± 11 | 150 ± 15 |
| MCP-1/LMhly− | 153 ± 15 | 157 ± 16 |
| IL-6/LMhly− | 86 ± 2 | 81 ± 4 |
| TNF-α/LMHKLM | 310 ± 11 | 321 ± 15 |
| MCP-1/LMHKLM | 123 ± 15 | 133 ± 16 |
| IL-6/LMHKLM | 156 ± 2 | 131 ± 4 |
| TNF-α/LPS | 3004 ± 35 | 3400 ± 31 |
| MCP-1/LPS | 3012 ± 21 | 3500 ± 24 |
| IL-6/LPS | 2204 ± 20 | 2550 ± 31 |
a LIMP-2−/− or wild type mice (n = 5) were infected with 1× LD50 intraperitoneally for 3 days. The mice were sacrificed, and sera were collected for cytokine measurement by FACS analysis. The results are expressed as concentrations in pg/ml extrapolated from an internal standard curve. LAMP-1−/− concentration of cytokines was as follows: 3050 ± 17 for TNF-α, 2500 ± 20 for MCP-1, 4280 ± 35 for IL-6, 30 ± 5 for IL-12, 5.6 ± 2 for IL-10, and 165 ± 11 for IFN-γ.
b BM-DM were infected with wild type LMwt, LLO-deficient mutant LMhly−, or heat-killed bacteria, LMHKLM (ratio 10:1 of bacteria: cell) for 1 h or treated with LPS from E. coli (1 ng/ml) and supernatants collected after 24 h. Cytokine measurement was performed as above.