TABLE 2.
Activation parameters and markers elicited by LM infection
| BM-DM activation response | LIMP-2−/− | Wild type |
|---|---|---|
| Oxybursta | 77.5 | 78.2 |
| Control isotypeb | 0.18 | 0.18 |
| CD11b | 22.3 | 21.1 |
| CD11c | 8.5 | 10 |
| Gr1 | 0.33 | 0.33 |
| IAb | 27.5 | 57.2 |
| F4/80 | 2.1 | 23 |
| CD54 | 67.4 | 89.9 |
a Oxidative burst capacity of BM-DM using the Phagoburst fluorescent reagent after 10 min of incubation with LM as stimulus and according to the instructions of the supplier. The results correspond with the fluorescent intensity mean of 100,000 cells using the CellQuest software program of data acquisition and analysis.
b BM-DM were also surface-labeled with specific monoclonal antibodies phycoerythrin-labeled. The percentages reflect the amount of positive cells for a given marker or stimulus.