TABLE 3.
Subcellular compartments for LM growth in different BM-DM genotypes
| BM-DM genotypesa | Phagosomal |
Cytosolic |
||
|---|---|---|---|---|
| 0 h | 8 h | 0 h | 8 h | |
| % | % | |||
| Ctsd+/+ | 32 | 2 | 68 | 98 |
| Ctsd−/− | 12 | 70 | 88 | 30 |
| LIMP-2−/− | 16 | 20 | 84 | 80 |
| LIMP-2+/+ | 33 | 1 | 67 | 99 |
a BM-DM from LIMP-2−/− or WT mice were infected with LM at a 10: 1 (cell: bacteria) ratio as described under “Experimental Procedures.” Phagosomal and cytosolic fractions were purified from PNS (30 μg) and solubilized, and viable LM were quantified as CFU to calculate the percentages of phagosomal and cytosolic bacteria as previously reported (15, 16). The results are expressed as percentages of total CFU internalized in PNS as described under “Experimental Procedures.” CFU values for PNS at 0 h were: 6.5 ± 0.03 for Ctsd+/+, 32.5 ± 0.1 for Ctsd−/−, 63.7 ± 0.01 for LIMP-2−/−, and 7.0 ± 0.03 for LIMP-2+/+.