FIGURE 3.

RBX1 silencing induces the accumulation of CDT1 and ORC1 but not oncoproteins. U87 cells transfected with siRBX1 or siCONT for 24 h were split. At 120 h post-splitting, cells were subjected to Western blot analysis for the indicated proteins with β-actin as the loading control (A). U87 (B) or H1299 (C) cells were transfected with siRBX1 or siCONT for 24 h, followed by cell splitting. At the indicated time points post-splitting, cells were harvested and subjected to Western blot analysis with β-actin as the loading control.