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APOBEC3G purification and activity. A, image of Coomassie Blue-stained A3G and A3G-H248A/H250A-Myc-His proteins purified from HEK-293T cells. A BSA standard is shown for comparison. B, DNA deamination activity of the indicated proteins (arbitrary fluorescent units). C, EMSA for the indicated proteins bound to a 43-nt ssDNA.
