FIGURE 7.
Enhancement of C4 and C3 deposition by MBL-PTX3 or SAP complex formation on C. albicans. MBL-PTX3 or MBL-SAP complexes were first established on C. albicans as described above before the addition of 10% MBL−/SAPdepleted serum. C4 and C3 deposition were assessed. As controls, the addition of MBL and PTX3 alone was applied. The inset shows depletion of SAP from MBL− serum using DNA-cellulose. Lane 1, before depletion; lane 2, after depletion. The MFI was used to assess C4 and C3 deposition. Results are presented as the mean ± S.E. of samples analyzed in triplicate. Results are representative of three independent experiments. The asterisks indicate the statistical significance versus controls: **, p < 0.01.