FIGURE 8.
C1q-dependent enhancement of C4 and C3 deposition by MBL-PTX3 complexes on C. albicans. MBL was depleted from C1q− serum and assessed by FACS (A) and Western blot (B), then SAP was depleted and assessed by Western blot (C). Lane 1, before depletion; lane 2, after depletion. The MBL-PTX3 complex was first established on C. albicans as described above followed by induction of C4 and C3 deposition in 10% C1q−/MBL and SAPdepleted serum with or without exogenous C1q. D, and E, C4 and C3 deposition were assessed by FACS. As controls, the addition of MBL, PTX3 alone, or mixtures of both were applied. The MFI was used to assess C4 and C3 deposition. Results are presented as the mean ± S.E. of samples analyzed in triplicate. Results are representative of three independent experiments. The asterisks indicate the statistical significance versus controls: **, p < 0.01.