C1q-dependent enhancement of phagocytosis by human neutrophils. A, complement dependent-phagocytosis of C. albicans by human neutrophils is shown. C. albicans were labeled with FITC and preincubated with or without MBL, PTX3, or SAP or a combination of the molecules to induce complement activation in MBL−/SAPdepleted serum. B, effect of C1q on phagocytosis of C. albicans induced by MBL-PTX3 complex is shown. C1q−/MBL and SAPdepleted serum was used as a complement source to opsonize FITC-labeled C. albicans after incubation with or without MBL, PTX3, SAP, C1q, or a combination of the molecules. Phagocytosis was analyzed by FACS. The bars represent values expressed as the average opsonophagocytosis ± S.E. of six donors (A) or three donors (B) measured on three different days. The asterisk indicates the statistical significance versus controls: *, p < 0.05.