TABLE 2.
SEC-MALS data
Indicated in the last column (oligomeric state) is the stoichiometry determined.
| Protein | Concentration | Elution volume | Measured mass | Theoretical mass | Oligomeric state |
|---|---|---|---|---|---|
| μm | ml | Da | Da | ||
| PDZ12 | 233 | 17.2 | 21,640 ± 0.8% | 21,307 | Monomer |
| PDZ23 | 233 | 16.3 | 26,970 ± 0.6% | 27,052 | Monomer |
| PDZ123 | 233 | 15.5 | 36,800 ± 0.3% | 36,922 | Monomer |
| E6 | 500–1340 | Variable | 99,000–271,000 | 8,759 | Higher order oligomers |
| E6 | 890 | 15.5 | 13,260 ± 35% | 8,759 | Monomer:dimer equilibrium |
| PDZ123:E6 (1:2.25) | 117:263 | 15.7 | 55,050 ± 0.4% | 54,439 (1PDZ:2E6) | 1 PDZ:2E6 |
| PDZ123:E6 (1:3.0) | 120:360 | 15.7 | 61,960 ± 2% | 63,198 (1PDZ:3E6) | 1 PDZ:3E6 |