TABLE 3.
Rate and equilibrium constants for the reaction between different PDZ domains and E6WT and E6ML151V, respectively
| Protein | kon | koffa | koff1b | koff2b | Kd (koff/kon) |
|---|---|---|---|---|---|
| μm−1s−1 | s−1 | s−1 | s−1 | μm | |
| E6WT-PDZ | |||||
| PDZ1 | 12 ± 1.8 | 12.0 ± 5.0 | 1.0 ± 0.5a | ||
| PDZ2 | 8.4 ± 1.0 | 34.0 ± 6.0 | 4.0 ± 0.9a | ||
| PDZ3 | 7.7 ± 1.0 | 16.0 ± 1.5 | 2.0 ± 0.3a | ||
| E6L151V-PDZ | |||||
| PDZ1 | 6.9 ± 0.3 | 4.7 ± 0.5 | 6.2 ± 0.5 | 0.9 ± 0.08b | |
| PDZ2 | 6.7 ± 0.5 | 5.2 ± 0.4 | 2.5 ± 0.3 | 0.4 ± 0.06b | |
| PDZ3 | 9.7 ± 0.2 | 2.7 ± 0.2 | 1.0 ± 0.2 | 0.1 ± 0.02b | |
| PDZ12 | 8.7 ± 0.2 | 7.5 ± 0.4 | 4.6 ± 0.4 | 1.3 ± 0.4 | |
| PDZ23 | 25 ± 0.4 | 4.3 ± 0.5 | 2.0 ± 0.4 | 0.5 ± 0.2 | |
| PDZ123 | 27 ± 1.2 | 9.3 ± 1.4 | 1.9 ± 0.5 | 0.7 ± 0.2 | |
a koff determined from Equation 3.
b koff determined from displacement experiments.