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Thermal shift assay of UmuD protein stability. Melting was observed by monitoring Sypro Orange fluorescence as a function of temperature. A, shown is a melting profile of wild-type UmuD2 (24) (green) and UmuD N41D (orange). B, change in calculated melting temperature between wild-type UmuD′2 (24) (navy blue) and UmuD′ N41D (magenta) variant protein at 45 μm is 6.0 °C.
