FIGURE 7.

Effect of COG112 on mRNA expression of iNOS and chemokines in colonic epithelial cells isolated from DSS-treated colons. Mice received 4% DSS or water alone (Ctrl) for 7 days. Mice were injected daily with COG112 (1 mg/kg) or PBS vehicle. Colons were harvested, and epithelial cells were isolated and positively selected with antibody to E-cadherin using magnetic beads (supplemental Fig. S3). mRNA was extracted and real-time PCR was performed for iNOS (A), MIP-2 (B), and KC (C) standardized to β-actin using SYBR green. *, p < 0.05; **, p < 0.01 versus control; §§, p < 0.01 versus DSS alone. n = 4 mice per group.