FIGURE 2.
Knockdown of p63α and β isoforms results in an increase in markers of EMT and invasion. A, protein levels of E-cadherin, vimentin, Slug, Snail, and Twist in p-Super retroviral MCF10A cells depleted of p53, all p63 isoforms (DBD) or p63α and β (UTR) using retroviral transduced shRNA (right panel) or depleted p63α and β using siRNA (UTRsi, right panel). Protein loading: mouse anti-actin antibody. B, qRT-PCR analysis of Slug, Snail, and Twist mRNA expression levels. C, invasion analysis of MCF10A retroviral transduced cell lines, showing significant increase in invasion in cells with UTR shRNA. All experiments were carried out in triplicate, and three independent studies were performed. Paired t tests were performed (B and C), and significant differences with respect to the control, SCR, are shown (*, p < 0.05; **, p < 0.001).