FIGURE 3.

Snail is not involved in the EMT phenotype. A, phase-contrast images of SCR and UTR control cells and cells overexpressing Snail, showing that there is no change in phenotype. B, protein levels of pan-TGFβ, E-cadherin, vimentin, Slug, Snail, and Twist analyzed by Western blotting in cells from A. The levels showed no change in morphology when Snail was exogenously expressed. C, phase-contrast images of SCR and UTR control cells and cells with a knockdown in Snail, showing that there is no difference in phenotype. D, protein levels of pan-TGFβ, E-cadherin, vimentin, Slug, Snail, and Twist analyzed by Western blotting, in cells from C. There was no change in morphology when Snail was depleted (β-actin detected using the mouse anti-actin antibody). All experiments were carried out in triplicate, and three independent studies were performed.