FIGURE 6.

Rescue of epithelial phenotype by reexpression of p63α. A, phase-contrast images of SCR and DBD with and without addition of adenoviral expressing ΔNp63γ (ΔNγ) isoform, showing that ΔNp63γ can induce an EMT phenotype. B, protein levels of Slug, Snail, and Twist analyzed by Western blotting in cells from A. C, phase-contrast images of SCR and UTR cells with and without addition of adenoviral ΔNp63α (ΔNα) isoform, showing that ΔNp63α can rescue an epithelial phenotype. D, protein levels of Slug, Snail, and Twist analyzed by Western blotting, in cells from C. β-Actin was detected using the mouse anti-actin antibody. All experiments were carried out in triplicate, and three independent studies were performed.