FIGURE 4.

Inhibition of adhesion to human chondroadherin by the peptide CQLRGLRRWLEAK³¹⁸. 105KC cells (A and B) and T47D cells (C) pretreated with 100 units/ml collagenase or primary bovine chondrocytes (D) pretreated with 100 units/ml collagenase and 10 units/ml hyaluronidase were added to 48-well plates coated with human chondroadherin expressed in E. coli, human chondroadherin expressed in EBNA cells, the CQLRGLRRWLEAK³¹⁸ peptide (striped bars) or collagen type II at 50,000 cells/well in the presence (white bars)or absence (gray bars) of the synthetic peptide (250 μg/ml, 150 μm for 105 kc cells and T47D cells, 33 μg/ml, 20 μm for primary bovine chondrocytes). The cells were allowed to adhere for 1 h at 37 °C, and non-adherent cells were removed by washing. Adhesion was quantified by analyzing lysosomal N-acetylglucosaminidase. Adhesion is expressed as number of cells, where the data represent the average of two wells. Error bars, S.D. in one experiment. Independent experiments showed similar results.