Fig. 2.

Immunofluorescence labeling (Texas red) of Apoptosis Inducing Factor (AIF) in medullary interstitial cells (MICs) under isotonic and hypertonic conditions (400 and 600 mosmol/L for 12 hours). Preincubation of MICs with 1 mmol/L betaine followed by exposure to 600 mosmol/L for 12 hours markedly decreased AIF staining (right lower quadrant). AIF/β-actin ratio was determined by western blot in MICs exposed to 400 and 600 mosmol/L for 12 and 24 hours respectively. All values are mean ± SEM of n=4; *p<0.05 compared to isotonic controls.