Figure 3.
Conformational changes in Polη ternary complex. a, Conformational change in Polη upon complex formation. Upon DNA and dNTP binding, the PAD swings by as much as ~13.6A towards the major groove of the DNA. The thumb also rotates towards the DNA but to a much lesser extent than the PAD. b, Comparison between our Polη ternary complex and that reported by Alt et al between Polη and a cisplatin adduct19. In the Alt et al. structure, Polη has an apo-like conformation (cf Figure 3a), wherein the PAD occupies a position similar to that in the apo Polη structure