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. 2011 Jan 21;8:3. doi: 10.1186/1743-8977-8-3

Figure 4.

Figure 4

Effects of MWCNT on macrophages in vitro - viability and mRNA content. Panel a: Cell viability assessed using the MTT assay. Viability was expressed as the percentage of control cell values after 24 h exposure to 100 μg/ml CB, crocidolite, or NT1, NT2, and NT3 in doses of 0.1 to 100 μg/ml. Abbreviations are the same as in Figures 1 and 2. Results are represented as box and whisker plots of values from 3-6 experiments. *: p < 0.05 vs. control condition. Panel b: HO-1 mRNA expression in RAW 264.7 cells after 6 h exposure to culture medium alone, 100 μg/ml CB, crocidolite, or NT1, NT2, and NT3 in doses of 0.1 to 100 μg/ml. Results are expressed as the ratios over RPL13 mRNA levels and represented as box and whisker plots of values from 3-6 experiments. Abbreviations are the same as in Figures 1 and 2, *: p < 0.05 vs. control condition. Panel c: CXCL2 mRNA expression in RAW 264.7 cells after 24 h exposure to culture medium alone, 100 μg/ml CB, crocidolite, or NT1, NT2, and NT3 in doses of 0.1 to 100 μg/ml. Results are expressed as ratios over RPL13 mRNA levels and represented as box and whisker plots of values from 3-6 experiments. Abbreviations are the same as in Figures 1 and 2, *: p < 0.05 vs. control condition, #: p < 0.05 vs. NT2.