. 2011 Jan 13;11:12. doi: 10.1186/1471-2229-11-12

Table 2.

RT-qPCR expression analysis of 32 stress-related genes showing significant differential expression in tissues from sugarcane variety Q117.

Gene	Mean
	P value	LFE¹	I _4-5	I _7-8	I _13-14

Up-regulated Trehalase	<.001	0.0102^a	0.0230^b	0.0240^b	0.0370^c
TAP24F-4	<.001	0.1241^a	1.1617^c	0.7071^b	0.6160^b
TM89-33	0.002	0.0659^a	0.1502^c	0.1059^b	0.1418^c
*TF1*²	0.009	0.0395^a	0.1370^b	0.1238^b	0.1866^b
TM11b	0.0342	0.0105^a	0.0217^b	0.0195^ab	0.0250^b
*Pox*	<.001	0.1511^ab	0.1127^a	0.1879^b	0.2798^c
*OAT*	<.001	0.0205^a	0.0199^a	0.0201^a	0.0872^b
AS	<.001	0.0126^a	0.1024^b	0.1159^b	0.1591^c
Samsynt	<.001	1.9011^a	11.9409^b	10.8635^b	13.0849^b
SPDS	0.0108	0.0785^a	0.0744^a	0.0935^ab	0.1056^b
PST2a	<.001	0.0566^a	3.2513^b	3.4418^b	2.5815^b
PST2b	0.008	0.0369^a	0.1816^b	0.2064^b	0.1904^b
*PST5*	0.021	0.0141^a	0.0688^ab	0.1428^bc	0.2272^c
PST7	<.001	0.0577^a	0.2210^c	0.1330^b	0.1302^b
*LEA*	<.001	0.0415^a	0.0755^a	0.0826^a	0.3197^b
*Dehydrin*	0.0442	0.0074^a	0.0158^a	0.0088^a	0.0454^b
ABC transporter	0.025	0.0252^a	0.0490^ab	0.0408^a	0.0665^b
*P5CS*	0.056	0.1093^a	0.1424^a	0.1465^a	0.2511^a
Down-regulated Gols	0.009	0.1009^b	0.0571^a	0.0172^a	0.0092^a
TPP	0.003	0.8318^b	0.4294^a	0.3904^a	0.3016^a
DREB-like protein	0.02	0.0016^a	0.0057^b	0.0022^a	0.0019^a
THB43-11	<.001	0.3665^b	0.1105^a	0.1361^a	0.1502^a
HvDRF1	<.001	0.4607^c	0.2112^b	0.1223^a	0.1409^a
TWC1	<.001	0.0529^b	0.0280^a	0.0200^a	0.0206^a
ShSUT1	0.001	0.1434^b	0.1261^b	0.0697^a	0.0481^a
DnaJ	<.001	0.2349^a	0.9842^c	0.6780^b	0.4610^ab
HPPase	<.001	0.0105^a	0.3023^b	0.0350^a	0.0270^a
Osmotin	0.003	0.0503^a	0.2302^b	0.0572^a	0.0577^a
Stress-related protein	0.002	0.0997^a	0.3784^b	0.1460^a	0.1152^a
Expansin	<.001	0.3810^a	4.1719^b	0.1148^a	0.0389^a
Lipoxigenase	<.001	0.0013^b	0.0002^a	0.0002^a	0.0003^a
PEAMT	0.016	0.2207^b	0.2079^b	0.0979^a	0.0926^a
ADI	<.001	0.0153^a	0.0251^c	0.0194^b	0.0181^ab

¹The tissues are as described in Table 1. The expression of each gene was normalised relative to that of GAPDH.

²The eight genes selected for analysis in further experiments are shown in bold typeface. (See additional file 2 for full name of each gene and primers sequences). Data for PC5S (P = 0.056) which was included in further experiments (see text) is also presented.

^aMean values within a gene with the same letter are not significantly different based on LSD test (P ≥ 0.05) from one-way analysis of variance (ANOVA).