Table 5. Titration of anti-FMDV serotype O antibodies induced following infection with rescued serotype O FMDV.
Calves | ||||||
dpi | 2C1 | 2C2 | 2C3 | 2C4 | 2C5 | 2C6 |
0 | - | - | - | - | - | - |
1 | - | - | - | - | - | - |
2 | - | - | - | - | - | - |
3 | - | - | - | - | - | - |
4 | - | - | - | - | - | - |
5 | 20 | - | - | - | 10 | N.D. |
6 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
7 | 320 | 10 | <10 | 10 | 160 | N.D. |
8 | 320 | 40 | 40 | 40 | 160 | N.D. |
9 | 320 | 80 | 40 | 80 | 80 | N.D. |
10 | 640 | 80 | 80 | 160 | 160 | N.D. |
Values are reciprocals of highest serum dilutions yielding positive results in the blocking ELISA on the indicated day post inoculation (dpi). A (-) indicates samples which were scored negative in the spot test (see Figure 5B) and N.D. indicates when samples were not collected (calf 2C6 was euthanized on day 4). Calves 2C1 and 2C5 were inoculated with serotype O FMDV (O/ISL/PAK/L1573/2009) on day 0 and the other calves were maintained in contact with them.