Figure 1. Representative flow cytometric map of CD4⁺ T cells isolated from peripheral blood of active VKH patients.

The PBMC and freshly isolated CD4⁺ T cells were stained with the indicated markers using fluorescence-labeled mAb and analyzed by flow cytometry. Before sorting, the ratio of CD4⁺ T cells in PBMC was 38.11% (A). After microbeads based sorting, the purity of CD4⁺ T cells was as high as 98.06% (B).