Table 2. The differently expressed proteins identified based on two or more peptides in CD4+ T cells between active VKH patients and normal individuals.
Gene symbol | Swiss-prot accession No. | Identified proteins | Expression Ratioa (VKH:NC) | Xcorrb | t-test p | MH+ (Da) | Mass stdev (Da) | Identified Peptide |
Up-regulated in VKH | ||||||||
VIM | B0YJC4 | Vimentin | 2.8 | 2.42 | 0.00004 | 1060.7690 | 0.08313 | 159 R.QVDQLTNDK.A 169 |
5.53 | 0.00002 | 1169.9804 | 0.11453 | 129 K.ILLAELEQLK.G 140 | ||||
2.27 | 0.00061 | 1428.8390 | 0.13478 | 50 R.SLYASSPGGVYATR.S 65 | ||||
3.16 | 0.00031 | 1115.8539 | 0.14490 | 105 K.VELQELNDR.F 115 | ||||
3.36 | 0.00032 | 1323.8875 | 0.13155 | 196 R.EEAENTLQSFR.Q 208 | ||||
3.99 | 0.00045 | 1406.1375 | 0.30727 | 223 K.VESLQEEIAFLK.K 236 | ||||
3.30 | 0.00030 | 1533.9759 | 0.26803 | 222 R.KVESLQEEIAFLK.K 236 | ||||
3.30 | 0.00029 | 1312.8985 | 0.35813 | 207 R.QDVDNASLAR.L 218 | ||||
2.26 | 0.00061 | 1226.9591 | 0.20369 | 294 K.FADLSEAANR.N 305 | ||||
KRT10 | P13645 | Keratin, type I cytoskeletal 10 | 2.0 | 2.26 | 0.00449 | 993.8803 | 0.14940 | 237 K.YENEVALR.Q 246 |
2.49 | 0.00409 | 1435.8816 | 1.01701 | 439 K.IRLENEIQTYR.S 451 | ||||
3.40 | 0.00108 | 2083.0051 | 0.17698 | 422 R.AETECQNTEYQQLLDIK.I 440 | ||||
2.27 | 0.02983 | 1262.8610 | 0.14280 | 450 R.SLLEGEGSSGGGGR.G 465 | ||||
3.03 | 0.00403 | 1031.5372 | 0.57250 | 257 R.VLDELTLTK.A 267 | ||||
3.84 | 0.00012 | 1494.0186 | 0.19081 | 322 R.SQYEQLAEQNRK.D 335 | ||||
2.26 | 0.00448 | 1236.916 | 0.16415 | 165 R.ALEESNYELEGK.I 178 | ||||
ATP5B | P06576 | ATP synthase subunit beta, mitochondrial precursor | 1.8 | 2.26 | 0.00433 | 1988.1477 | 0.17359 | 387 R.AIAELGIYPAVDPLDSTSR.I 407 |
3.57 | 0.00622 | 975.7282 | 0.15882 | 201 K.IGLFGGAGVGK.T 213 | ||||
2.67 | 0.00022 | 1088.6165 | 0.50286 | 188 K.VVDLLAPYAK.G 199 | ||||
ACTA2 | P62736 | Actin, aortic smooth muscle | 1.8 | 3.83 | 0.01604 | 1354.8744 | 0.12513 | 52 K.DSYVGDEAQSKR.G 65 |
3.12 | 0.00290 | 1198.7264 | 0.08676 | 52 K.DSYVGDEAQSK.R 64 | ||||
KRT2 | P62736 | Keratin, type II cytoskeletal 2 epidermal | 1.8 | 4.91 | 0.00099 | 1191.8102 | 0.09092 | 374 K.YEELQVTVGR.H 385 |
4.89 | 0.00012 | 1461.0514 | 0.45310 | 302 K.VDLLNQEIEFLK.V 315 | ||||
3.95 | 0.00025 | 1372.6575 | 0.48309 | 441 K.LNDLEEALQQAK.E 454 | ||||
KRT1 | P04264 | Keratin, type II cytoskeletal 1 | 1.6 | 2.24 | 0.00899 | 993.8803 | 0.14940 | 443 K.LNDLEDALQQAK.E 456 |
4.70 | 0.00027 | 1435.8816 | 1.01701 | 343 R.SLDLDSIIAEVK.A 356 | ||||
3.08 | 0.00072 | 2083.0051 | 0.17698 | 185 K.SLNNQFASFIDK.V 198 | ||||
ALB | A6NBZ8 | Uncharacterized protein ALB | 1.6 | 2.95 | 0.04948 | 1444.1611 | 0.54524 | 286 K.YICENQDSISSK.L 299 |
3.08 | 0.00704 | 1512.1173 | 0.04618 | 438 K.VPQVSTPTLVEVSR.N 453 | ||||
2.95 | 0.04947 | 1078.9591 | 0.10821 | 499 K.CCTESLVNR.R 509 | ||||
Down-regulated in VKH | ||||||||
SFRS1 | Q07955 | Isoform ASF-1 of Splicing factor, arginine/serine-rich 1 | 0.50 | 2.52 | 0.00007 | 1255.5932 | 0.37017 | 17 R.IYVGNLPPDIR.T 29 |
2.78 | 0.03060 | 1078.9591 | 0.10822 | 154 R.DGTGVVEFVR.K 165 | ||||
3.37 | 0.00126 | 1417.7458 | 0.11825 | 142 R.EAGDVCYADVYR.D 155 |
a: average expression ratio;
b: cross-correlation score.