Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jan 28;6(1):e16307. doi: 10.1371/journal.pone.0016307

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Mattiuzzo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Kinetochore accumulation of CENP-E (red) in an early and a late normal prometaphase from vector transfected cultures and in a disorganized prometaphase and a late prometaphase with alignment defects from EGFP-Hec1 (green) trasfected cultures. Kinetochores are marked by CREST staining (blue). The graph shows a quantitative analysis of CENP-E intensity at kinetochores, in vector and EGFP-Hec1 transfected cells. CENP-E/CREST ratio was significantly higher in disorganized prometaphases (P<0.01, t-test) and in prometaphases with alignment defects (P<0.05) from EGFP-Hec1 expressing cells than in normal late prometaphases from vector transfected cells. (B) Chromosome congression and spindle organization in control (siGL2) or CENP-E silenced (siCENP-E) mitoses or mitoses expressing EGFP-Hec1 after control silencing (siGL2+ EGFP-Hec1) or CENP-E silencing (siCENP-E + EGFP-Hec1). Mitoses were observed after α-tubulin (blue) and CENP-E (red) staining. Kinetochores were identified by CREST staining in siGL2 and siCENP-E samples or by EGFP expression in induced samples (green). The graph shows the frequencies of abnormal PM in the different conditions. Data are mean ± SE of >150 PM scored for each condition in two experiments. Bars, 5 µm. (C) KT-MT attachments in an EGFP-Hec1 (green) expressing disorganized bipolar prometaphase exposed to MG132 for 2 h and stained for CENP-E (red) and α-tubulin (blue) after calcium buffer treatment. A maximum projection from deconvolved z-stacks is shown. Insets show a 3-fold enlargement of one optical slice from the boxed regions. An end-on attachment (inset 1) and a side-on attachment (inset 2) are shown.