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. 2011 Jan 28;6(1):e16477. doi: 10.1371/journal.pone.0016477

Figure 3. p38α activity is required for BMP-2-induced cell migration.

Figure 3

(A) Immunoblot analysis of p38α, β, γ and δ isoform expression in p38α -/- MEF cells. (B) p38α -/- MEF cells were treated with BMP-2 and SB203580 as indicated and cell lysates were analysed by immunoblotting with anti-phospho-p38, anti-p38α, anti-phospho-MK2, anti-phospho-Hsp27 and anti-Tubulin antibodies. MK2 was also immunoprecipitated and subjected to an in vitro kinase assay with GST-Hsp25 as a substrate. Relative kinase activities are expressed as mean +/- S.E.M. of three independent experiments. (C) Wounded wt or p38α -/- MEF monolayers were allowed to migrate for 24 h in the presence or absence of BMP-2. Histogram shows the quantitative analysis of invaded area and values correspond to the mean ± SEM from three independent experiments (*, p<0.001 compared with the corresponding condition in absence of BMP-2, One-way ANOVA followed by Bonferroni's multiple comparison test).