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. 2011 Jan 28;6(1):e16477. doi: 10.1371/journal.pone.0016477

Figure 5. BMP-2 activates p38 independently of Cdc42/PI3K pathways.

Figure 5

(A) C2C12 cells transfected with mock plasmid or GFP-tagged wild-type Cdc42 (GFP-Cdc42wt) or a dominant-negative form (GFP-Cdc42N17) were stimulated with BMP-2 and cell lysates were analyzed by immunoblotting with anti-phospho-p38 and anti-phospho-Hsp27 antibodies. The membranes were stripped and retested with anti-GFP, anti-Cdc42 and anti-Tubulin antibodies. Bands corresponding to GFP-Cdc42 overexpressed forms and endogenous Cdc42 are indicated by asterisks. (B) C2C12 cells transfected with GFP or myc-tagged plasmids for a constitutive active form of PAK1 (Pak1-HL) or a dominant-negative form (Pak1-PID) were stimulated with BMP-2 and cell lysates were analyzed by immunoblotting with anti-phospho-p38 and anti-phospho-Hsp27 antibodies and reprobed with anti-myc or anti-Tubulin antibodies. The anti-phospho-Hsp27 antibody recognizes several unspecific bands, but only the one indicated with an asterisk corresponds to the phosphorylated form of Hsp25.