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. 2011 Jan 28;6(1):e16477. doi: 10.1371/journal.pone.0016477

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Gamell et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) C2C12 cells were treated with BMP-2 and SB203580 as indicated and cell lysates were analysed by immunoblotting with anti-phospho-LIMK1 and anti-Tubulin antibodies. (B) C2C12 cells were treated with BMP-2 and SB2035380 as indicated and endogenous LIMK1 was immunoprecipitated and subjected to an in vitro kinase assay with GST-cofilin as a substrate.