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. 2011 Jan 28;6(1):e16607. doi: 10.1371/journal.pone.0016607

Figure 4. Bareback-processing increases sequencing depth without introducing bias.

Figure 4

(a) The percentage of sequences containing the expected initially biased sequence (IBS) identifiers, processed with either the standard SCS pipeline or subjected to bareback-processing, shuffling the first four bp to the end of the sequence. (b) Comparison of the sequencing depth in a 14 kb region for two- and four-IBS library samples after SCS- or bareback-processing, followed by sequence alignment. Each bar represents a window of 100 bp, and the heat map colours range between 20 and 500 sequencing reads. (c) Scatter plot representation comparing the read count distribution of SCS- vs. bareback-processed samples with either two or four different IBS. Reads of an entire flow-cell lane were counted in sliding windows of 100 bp.