Figure 2. CEppt alleviates toxic effects of Aβ – cell and fly assays.

(A) Samples of Aβ₄₂ (20 µg/ml) with or without various concentrations of CEppt were incubated for 24 hours with PC12 cells culture. To exclude any toxic effect of CEppt, CEppt alone (9 µg/ml) was pre-incubated for 24 hours with PC12 cells (ref CEppt). Cells viability was determined using MTT viability assay. ** Pv<0.005. (B) The effect of CEppt on longevity of Aβ₄₂-expressing flies. The life span of four classes of flies was evaluated. AD flies grown on regular medium (black line), AD flies grown on medium containing CEppt (black dashed line), Control Flies (carrying the Aβ₄₂ transgene but not expressing it) grown on regular medium (grey line), Control Flies (carrying the Aβ₄₂ transgene but not expressing it) grown on medium containing CEppt (grey dashed line). (C) The effect of CEppt on the climbing behavior of Aβ₄₂-expressing flies. Four classes, each containing six-nine vials with 10 flies in each: AD flies grown on regular medium (black),AD flies grown on medium containing CEppt (grey), Control flies (carrying the Aβ₄₂ transgene but not expressing it) grown on either regular medium (striped) or on medium containing CEppt (white), were analyzed using the climbing assay. Results show the percent of flies climbing to the top of the vial after 18 seconds, during the course of 10 days. Pv<0.0001. (D) Head extract from 4 days old flies. OR-control flies (left), AβArc₄₂-expressing flies unfed (middle) fed with 0.75 mg/mL CEppt (right) (n = 40 in each group). Accumulation of Aβ tetramers is evident only in AβArc₄₂ flies which were not fed with CEppt.